Fig. 6.

Linc01296 facilitates the O-glycosylation on MUC1 and activates PI3K/AKT pathway. a CRC cells were treated with GALNT3 or shGALNT3. Total MUC1, VVA-binding MUC1 and GALNT3 levels were analyzed by western blot. b Western blot of MUC1 and VVA blocking on activation of PI3K/AKT cascade. c The activity of PI3K/AKT cascade was measured with GALNT3 or shGALNT3 treatment. d The 5-FU resistant CRC cells were pretreated with DMSO or LY294002. The PI3K/AKT pathway molecules were analyzed by western blot. e With the mediation of miR-26a and linc01296, the main molecular levels of PI3K/AKT cascade were detected by western blot