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. 2018 Dec 14;18:214. doi: 10.1186/s12866-018-1343-0

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — In silico simulation of amplification and taxonomic assignment. a Neighbor-joining tree of the full-length ITS sequences included in the custom database used in the simulation. b Barplots showing the number of sequences predicted to be amplified by each primer set at different combinations of mismatches. H: primer head. T: primer tail. Numbers correspond to the number of mismatches either in H or T. c Barplots showing the number of false negative (FN), false positive (FP), and true positive (TP) sequences with each primer set. d Percentage of sequences per genus correctly assigned with each primer set at the different mismatch combinations