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. 2018 Dec 1;32(23-24):1562–1575. doi: 10.1101/gad.317362.118

Figure 3.

Figure 3.

Intestinal and hypodermal PFD-6 contributes to the long life span of daf-2 mutants. (A) pfd-6p::pfd-6::GFP was expressed in various tissues (panel a), including the intestine (panel b), the hypodermis (panel c), neurons (panel d), and muscles (panel e). Arrowheads indicate PFD-6::GFP in the intestine, hypodermis, neurons, or muscles. Bars, 50 µm. (B,C) The longevity of daf-2(e1370) [daf-2(−)] mutants was significantly decreased by intestine {rde-1(ne219); kbls7[nhx-2p::rde-1; rol-6D]}-specific pfd-6 RNAi (B) or hypodermis {rde-1(ne219); kzls9[lin-26p::nls::gfp; lin-26p::rde-1; rol-6D]}-specific pfd-6 RNAi (C). We noticed that life span-decreasing effects of the intestine- or the hypodermis-specific pfd-6 RNAi were greater than the life span-decreasing effects of pfd-6 RNAi on rde-1(−); daf-2(−) animals (Supplemental Fig. S3D). (D,E) Muscle {rde-1(ne219); kzls20[hlh-1p::rde-1, sur-5p::nls::gfp]}-specific (D) or neuron {sid-1(pk3321); uIs69[myo-2p::mCherry; punc-119::sid-1)]}-specific (E) pfd-6 RNAi did not affect the life span of daf-2(+) or daf-2(−) animals. We confirmed the efficiency of neuron- and muscle-specific pfd-6 RNAi knockdown using fluorescence-tagged PFD-6 proteins (Supplemental Fig. S3F–I). See Supplemental Table S4 for statistical analysis and additional repeats.

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