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. 2018 Dec 1;32(23-24):1537–1549. doi: 10.1101/gad.318436.118

Figure 1.

Figure 1.

NCoR1 and BAP1 are new members of the DRED complex. (A) A schematic representation for identification of new DRED complex proteins using the BioID strategy. (B) Anti-TR4 Western blotting of whole-cell lysates (Cui et al. 2011). Successful generation of an active TR4-BirA* fusion protein was confirmed by demonstrating the presence of a new shifted band (top arrow) in comparison with endogenous TR4 (bottom arrow). (C) Streptavidin staining of whole-cell lysates prepared from TR4-BirA* transformed HUDEP-2 cells. The arrowhead indicates that the TR4-BirA* fusion protein is self-biotinylated, and the asterisk indicates the presence of a background biotinylated band detected in all cell extracts. (D) Selected proteins identified in the TR4 complex. (#PSM) Peptide spectrum matches. The total number of identified peptide sequences representing each protein is shown. (E) Coimmunoprecipitation confirmation that NCoR1 forms complexes with TR4, LSD1, DNMT1, and histone deacetylase 1 (HDAC1) in HUDEP-2 cells.