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. Author manuscript; available in PMC: 2019 Aug 8.
Published in final edited form as: Cell Host Microbe. 2018 Jul 19;24(2):271–284.e3. doi: 10.1016/j.chom.2018.06.017

Figure 4.

Figure 4

(A) Stitched SD-IVM image of mouse liver at 8 h after i.v. infection with S. aureus GFP (5×107 CFU, USA300 LAC-GFP; bright green), autofluorescent hepatocytes (dull green) and platelets (CD49b; blue). Bar, 250 μm. (B) Quantification of platelet accumulation in the mouse livers at different time points after infection with wild-type S. aureus (USA300 LAC) or S. aureusΔhla. n = 4 per group. Data represents mean ± SEM. (C) Quantification of platelet accumulation in the mouse livers at different time points after infection with S. aureus in prophylactically treated mice with c-IgG or MEDI4893* mAb. c-IgG or MEDI4893* mAbs were i.v. administered at 30MPk 3h prior to AT intoxication. n = 4 per group. (B-C) Two- way ANOVA with multiple comparison. * P<0.05. Data represents mean ± SEM. (D) Quantification of blood platelet levels measured by flowcytometry at 8h post-infection with wild-type S. aureus or S. aureusΔhla (left) or prophylactically treated mice with c-IgG or MEDI4893* infected with wild-type S. aureus (right).Mice were injected with 30MPK mAbs at 2hrs prior to infection. n = 5 mice. *P<0.05. Data represents mean ± SEM.t-test.* p<0.05. (E) SD-IVM images of the liver 15 min after infection with wild-type S. aureus (USA300 LAC) or S. aureusΔhla in C57Bl/6 mice. S. aureus-GFP (bright-green), platelets (CD49b; blue). (F) Quantification of SD-IVM videos of mice infected with wild-type S. aureus (USA300 LAC) or S. aureusΔhla in C57Bl/6 mice. n = 4 mice Data represents mean ± SEM. Video 5 (G) SD-IVM images of the liver 15 min after infection with wild-type S. aureus-GFP in C57Bl/6, PF4ADAM10−/−or C3−/−-mice. (H) Quantification of platelet aggregation in mouse livers of SD-IVM images form (G) n = 4 mice; Line represents mean ± SEM. (I) Quantification of platelet accumulation in the mouse livers at 8h after infection with wild-type S. aureus-GFP in C57Bl/6, PF4ADAM10−/−or C3−/−-mice. n = 4 mice; Data represents mean ± SEM. One-way ANOVA with multiple comparison. ** P<0.01.