KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti - F4/80 (clone BM8) | Ablab | AbLab Cat. No.75– 0035–05 |
| Anti - CD49b (clone HMα2) | Biolegend | Biolegend Cat. No. 83598 |
| Anti - CD31 (clone MEC13.3) | Biolegend | RRID: AB_2161030 BioLegend Cat. No. 102515 |
| Anti - P-selectin (clone RP40.3) | BD Biosciences | RRID:AB_395026 BD Biosciences Cat.no. 553744, |
| Bacterial and Virus Strains | ||
| Staphylococcus aureus USA300 LAC | (Inoshima et al., 2011) | N/A |
| Staphylococcus aureus USA300 LACΔhla | (Inoshima et al., 2011) | N/A |
| Staphylococcus aureus USA300 LACΔagr | Periasamy et al., 2012) | N/A |
| Staphylococcus aureus USA300 LACΔαβhld | Periasamy et al., 2012) | N/A |
| Staphylococcus aureus USA400 MW2 | The Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) |
NARSA Cat.nr. NRS123 |
| Staphylococcus aureus USA100 | NARSA | NARSA Cat.nr. NRS382 |
| Staphylococcus aureus USA200 | NARSA | NARSA Cat.nr. NRS261 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Qdots 655 | ThermoFisher | Cat. Nr. Q21021MP |
| Recombinant alpha toxin (AT) | Medimmune (Cohen et al., 2016) | N/A |
| Recombinant non toxigenic ATH35L | Medimmune (Cohen et al., 2016) | N/A |
| MEDI4893* (Suvratoxumab) | Medimmune (Cohen et al., 2016) | N/A |
| Clodronate liposomes | Liposoma | Cat.Nr. CP-005–005 |
| Critical Commercial Assays | ||
| Mouse Haptoglobin ELISA Kit | Abcam | Cat.Nr. ab157714 |
| Experimental Models: Organisms/Strains | ||
| C57BL/6J | The Jackson Laboratory |
Stock No: 000664 |
| C3−/−; (B6;129S4-C3tm1Crr/J) | The Jackson Laboratory |
Stock No: 003641 |
| FcyR−/− B6;129P2-Fcer1gtm1Rav/J | The Jackson Laboratory |
Stock No: 002847 |
| VWF −/− B6.129S2-Vwftm1Wgr/J | Gift from D Lillicrap (Queens University) |
TheJackson Laboratory, Stock No: 003795 |
| GpIbα−/− | Gift from T. Chavakis (Dresden University of Technology) |
N/A |
| PF4 ADAM10−/− (PF4cre Adam10loxP/loxPmice) | (Powers et al., 2015). | N/A |
| Cd41-yfpki/ki | Gift from Kelly McNagny, University of British Columbia) |
N/A |
| Software and Algorithms | ||
| Volocity | PerkinElmer | N/A |
| Image J | National Institute of Health |
https://imagej.nih.gov/ij/ |
| Prism 7 | GraphPad | V7.02 |
| FlowJo | FlowJo | V10.1 |