Figure 5. Membrane TLR2 and CHRNA7 protein levels are reduced by nicotine treatment in MRSA infected diabetic mouse wounds.

(A) Expression of TLR2, CHRNA7, and β-actin in skin of control (Lepr^db/+) or diabetic (Lepr^db/db) mice at day 1 post wounding were detected by Western Blot (n=8 per group, repeated twice). C57Bl/6 mouse brain lysate was used as positive control. Semi-quantitative expression of membrane and soluble TLR2 (B) and CHRNA7 (C) was determined by densitometry after normalization to the internal control band (β-actin). Data are presented as the mean ± sem. Statistical analysis was performed using one way ANOVA and Bonferroni’s post-test adjusted P value (***, p < 0.001). Veh, vehicle treatment; Nic, nicotine treatment (10⁻⁹ M).