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. Author manuscript; available in PMC: 2019 Dec 15.
Published in final edited form as: Cancer Res. 2018 Nov 6;78(24):6785–6794. doi: 10.1158/0008-5472.CAN-17-3551

Figure 4.

Figure 4.

EGFR and EGFRvIII induce macrophage attraction through upregulating CCL2. A, Depletion of CCL2 in conditioned media from U87 cells expressing EGFR and EGFRvIII decreased attraction of MV-4–11 (antibody concentration: 1μg/ml). B, Knockout of CCL2 decreased attraction of MV-4–11. C, Kaplan-Meier curve of nude mice bearing intracranial xenografts of indicated cell lines. D, CCL2 expression levels were plotted according to EGFRvIII status in glioblastoma patient samples (TCGA). Red dots: 20 samples with highest expression of CCL2 mRNA. Blue dots: 20 samples with lowest expression of CCL2 mRNA. Macrophage attraction levels were normalized to the control cell line. Results were reproduced in three independent experiments with triplicate samples. ***, p< 0.001.