EGFR and EGFRvIII signal through KRAS to induce cytokine expression and macrophage attraction. A, Gene set enrichment analysis of genes upregulated by EGFR and EGFRvIII. B, Heat map of top 40 genes induced by EGFR and EGFRvIII in KRAS knockout U87 cells co-expressing EGFR and EGFRvIII. C, Real-time PCR. D, Knockout of KRAS decreased attraction of MV-4–11. Real-time PCR and macrophage attraction results were normalized to the control cell line. Results were reproduced in three independent experiments with triplicate samples.***, p < 0.001.