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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Exp Cell Res. 2018 Nov 22;374(1):128–139. doi: 10.1016/j.yexcr.2018.11.017

Fig. 4.

Fig. 4.

Opposing effects of integrin β3 and integrin β5 knockdown on VANGL2 protein levels.(A) Western blots of total protein from non-targeting (NT) control and integrin β3 (ITGB3) knockdown HT-1080 cells. Integrin β3 protein is reduced by 89%. (B) Box plot shows median value ± standard deviation (whiskers) and the interquartile range (boxes) of likely variation. Integrin β3 knockdown cells have increased VANGL2 protein levels (experiment performed three times, n = 9 biological replicates). (C) Western blots of total protein from NT control and integrin β5 knockdown cells. Integrin β5 protein is reduced by 100%. (D) Box plot showing integrin β5 knockdown cells have decreased VANGL2 protein levels (experiment performed three times, n = 8 biological replicates). *P < 0.05; **P < 0.01; P values are versus NT siRNA; two-tailed unpaired t-test.