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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Exp Cell Res. 2018 Nov 22;374(1):128–139. doi: 10.1016/j.yexcr.2018.11.017

Fig. 8.

Fig. 8.

Inhibition of cell-matrix adhesion reduces VANGL2 protein levels. (A) Box plot of HT-1080 vitronectin cell adhesion assay data showing effect and titration of RGD peptide treatment. The median value ± standard deviation (whiskers) and the interquartile range (boxes) are shown.(B) Western blots of untreated (UT) and RGD peptide treated cells plated on vitronectin (Vn) and fibronectin (Fn). (C) Box plot showing VANGL2 protein levels are decreased when integrin-vitronectin adhesion is inhibited (experiment performed four times, n = 10 biological replicates).**P < 0.01; ****P < 0.0001; P values are versus untreated cells; two-tailed unpaired t-test.