Table 3.
Fitting of the lipid mixing kinetics.
Membrane composition | Aβ40-to-lipid ratio | k (min−1) | % of lipid mixinga |
---|---|---|---|
DMPC/DMPS/cholesterol | 1:30 | 0.0028 (0.0004) | 20.55 (3.22) |
1:60 | 0.0023 (0.0003) | 47.12 (2.05) | |
1:90 | 0.0023 (0.0003) | 90.94 (2.07) | |
1:120 | 0.0022 (0.0003) | 88.70 (2.21) | |
DMPC/DMPS/cholesterol/sphingomyelin | 1:30 | 0.0060 (0.0005) | 4.32 (2.29) |
1:60 | 0.0051 (0.0003) | 16.48 (3.03) | |
1:90 | 0.0014 (0.0003) | 61.87 (2.61) | |
1:120 | 0.0014 (0.0003) | 66.76 (3.12) | |
DMPC/DMPS/cholesterol/sphingomyelin/ganglioside GM1 | 1:30 | –b | – |
1:60 | 0.0046 (0.0003) | 16.09 (2.22) | |
1:90 | 0.0059 (0.0004) | 17.06 (2.47) | |
1:120 | 0.0024 (0.0003) | 52.19 (3.05) |
Percentage of leakage was calculated as (I0 + I1)/Imax.
The increment of fluorescence was insignificant.