TABLE II.

Summary of RAG2 mutations and recombination activity levels

No.	Amino acid position	Domain affected by the mutation	Mutation	Mean recombination activity (% of WT hRAG2)	SEM
1	1	Core	M1T	65.3	2.2
2	16	Core	Q16X	1.7	0.4
3	35	Core	G35A	22.1	3.1
4	35	Core	G35V	0.4	0.3
5	39	Core	R39G	0.2	0.1
6	41	Core	C41W	0.2	0.4
7	62	Core	F62L	19.6	3.0
8	65	Core	D65Y	6.8	1.2
9	73	Core	R73H	12.4	1.4
10	77	Core	T77N	42.6	2.7
11	95	Core	G95R	0.3	0.2
12	110	Core	M110L	74.6	1.8
13	127	Core	K127X	0.1	0.0
14	157	Core	G157V	0.4	0.2
15	160	Core	S160L	5.8	0.6
16	180	Core	P180H	31.1	0.5
17	195	Core	Y195D	2.0	0.3
18	215	Core	T215I	67.2	1.0
19	229	Core	R229Q	8.9	1.0
20	229	Core	R229W	10.5	0.5
21	253	Core	P253R	95.4	2.3
22	278	Core	Q278X	0.1	0.1
23	285	Core	M285R	24.7	0.8
24	307	Core	W307X	0.2	0.2
25	386	Acidic	F386L	109.1	5.0
26	407	Acidic	E407X	2.9	0.4
27	416	PHD	W416L	1.4	0.2
28	437	PHD	E437K	0.9	0.2
29	440	PHD	K440N	26.7	2.4
30	443	PHD	M443I	0.4	0.2
31	444	PHD	I444M	2.7	0.3
32	446	PHD	C446W	2.9	0.1
33	451	PHD	G451A	66.3	4.8
34	453	PHD	W453R	0.6	0.1
35	456	PHD	A456T	16.0	2.9
36	459	PHD	M459L	30.8	0.6
37	474	PHD	N474S	97.5	5.9
38	478	PHD	C478Y	0.2	0.1
39	480	PHD	E480X	2.8	0.6
40	481	PHD	H481P	23.8	3.9
41	502	—	M502V	99.6	3.4

The activity of each RAG2 variant was determined by at least 3 independent experiments from which the mean value was calculated.

PHD, Plant homology domain.