Figure 1.

Confocal images of whole‐mount preparations showing close relationship between ICC and nNOS⁺ varicose nerve fibres in the murine proximal colon. Double labelling of the intramuscular class of ICC in colon (ICC‐IM) showing varicose processes of (A) nNOS⁺ neurons (NOS1; arrows) and (B) c‐Kit⁺ ICC‐IM (arrow heads). nNOS⁺ neurons track along ICC‐IM for distances greater than 250 μm and form close contacts. (C) Merged image showing the close relationship between nNOS⁺ neurons and ICC‐IM (arrows). Scale bar = 50 μm applies to all panels.