Figure 3.

Elimination of slow (nitrergic) IJP in colon of Prkg1 ^−/− mice. (A) Intracellular recording of IJP in murine proximal colon from a control [tamoxifen (TAM)‐treated c‐Kit^CreERT2/+;Prkg1^f/+] mouse. IJP, evoked by EFS, evoked by EFS, consisting of a fast IJP (fIJP) due to release of a purine, and a slow IJP (sIJP), due to release of NO. (B) IJP evoked in proximal colon of a TAM‐treated (3 days) c‐Kit^CreERT2/+; Prkg1^f/f mouse. Expression of iCre is limited to ICC in these mice. Note reduction of sIJP in panel (B). PKG1 was not resolved in 40% of cells impaled in the c‐Kit^CreERT2/+; Prkg1^f/f animals after 3 days of TAM treatment. Redrawn from Klein et al. (2013)