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. 2018 Dec 10;9:628. doi: 10.3389/fgene.2018.00628

FIGURE 5.

FIGURE 5

miR-125a directly regulates Wnt2. (A) Putative miR-125a-binding sites (red letters) and mutated sites (blue letters) in the 3′-UTR of rabbit Wnt2 in the pMir-report Dual-Luciferase miRNA Target Expression Vector. (B) Luciferase activity assays of RAB-9 cells co-transfected with Wnt2 3′-UTR-WT and a miR-125a mimics,Wnt2 3′-UTR-MUT and a miR-125a mimics. (C) Luciferase activity assays of RAB-9 cells co-transfected with Wnt2 3′-UTR-WT and a miR-125a inhibitors, Wnt2 3′-UTR-MUT and a miR-125a inhibitors. The activity of the firefly luciferase protein was normalized to Renilla luciferase activity. (D) The effect of miR-125a mimics on the endogenous Wnt2 gene expression in RAB-9 cells. (E) The effect of miR-125a inhibitors on the endogenous Wnt2 gene expression in RAB-9 cells. (F) Simple Western analysis of Wnt2 (40 kDa) expression when miR-125a was over-expressed or inhibited. GAPDH (36 kDa) as the internal control. NSB stood for non-specific binding. (G) The effect of miR-125a overexpression on the mRNA expression of CTNNB1, LEF-1, PPARD and TGFB1. (H) The effect of miR-125a inhibition on the mRNA expression of CTNNB1, LEF-1, PPARD and TGFB1. ∗P < 0.05, ∗∗P < 0.01.