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Time course of nicotine biodegradation (A) and growth (B) of P. putida J5 and the modABC mutants. All strains were grown in NI medium supplemented with 1 g/l nicotine and incubated at 30°C and 200 rpm. Culture samples were removed periodically to be measured for absorbance at 600 nm and the residual nicotine with HPLC. Presented data are averages and standard errors of the mean for at least three cultures that were assayed in duplicate.
