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Nicotine degradation of P. putida J5 was inhibited by tungstate. P. putida J5 was cultured in NI medium with or without 1 mM sodium tungstate at 30°C and 200 rpm. Nicotine-degrading efficiency was monitored every 2 h as described above. Presented data are averages and standard errors of the mean for at least three cultures that were assayed in duplicate.
