Table 1.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Genotype or relative phenotype | Source |
|---|---|---|
| Escherichia coli | ||
| DH5α | F- recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1Δ (argF-lacZYA)I169Φ80lacZ ΔM15 | Sambrook and Russell, 1998 |
| Pseudomonas putida | ||
| J5 | Apr; wild type | Wei et al., 2009 |
| M430 | Nicotine-degrading mutant inserted with Tn5 | This study |
| M728 | Nicotine-degrading mutant inserted with Tn5 | This study |
| M9502 | Nicotine-degrading mutant inserted with Tn5 | This study |
| ΔmodABC | modABC deletion mutant | This study |
| Arthrobacter oxidans | ||
| J4 | Nicotine-degrading bacterium | Lab collection |
| Plasmids | ||
| pBluescript II SK+ | ColE1 origin; Apr | Stratagene |
| pHSG299 | ColE1 origin; Kmr | TaKaRa |
| pRK2013 | ColE1 replicon with RK2 transfer region, helper plamsid; Kmr | Figurski and Helinski, 1979 |
| pRG970Km | Cloning vector containing promoterless lacZYA for construction of transcriptional fusions; Kmr | Yan et al., 2009 |
| pME6032 | Shuttle vector; Tcr | Heeb et al., 2000 |
| pBBR1MCS-2 | Shuttle vector; Kmr | Kovach et al., 1995 |
| pHSG299Δmod | pHSG299::modABC | This study |
| p6032-modABC-J5 | pME6032 with full length of modABC genes from J5 | This study |
| p6032-modABC-J4 | pME6032 with full length of modABC genes from J4 | This study |
| pMCS2-modABC-J5 | pBBR1MCS-2 with full length of modABC genes from J5 | This study |
| pMCS2-modABC-J4 | pBBR1MCS-2 with full length of modABC genes from J4 | This study |
| p970-PmodABC | pRG970Km with a 580 bp fragment containing the promoter of modABC genes | This study |