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. 2018 Dec 10;9:2901. doi: 10.3389/fimmu.2018.02901

Figure 3.

Figure 3

Th22 cells accumulate in inflamed synovial tissues. (A) Dual-labeling immunofluorescence staining [anti-IFN-γ, anti-IL-17, anti-IL-22 (green), anti-CD4 antibody (red), and nuclei staining with DAPI (blue)] of synovial tissues from patients with active RA (magnification: 200×). (B) Immunohistologic localization of Th1 cells (IFN-γ+ CD4+), Th17 cells (IL-17+ CD4+), and Th22 cells (IFN-γ− IL-17− IL-22+ CD4+) in synovial tissue from RA patients exhibiting low degrees of disease activity (LDA-RA), high degrees of disease activity (HDA-RA), or OA. Dual-labeling immunofluorescence staining was performed using anti-IFN-γ, anti- IL-17, anti-IL-22 (green), anti-CD4 antibody (red), and nuclei staining with DAPI (blue). Merged images are shown (top; magnification: 200×). The number of cells was counted. Each symbol represents one donor sample. Horizontal bar = mean; vertical bar = standard deviation. (C) Immunohistochemical analysis in sequenced slices of the synovial tissues from patients exhibiting LDA-RA, HDA-RA, or OA was performed using specific antibodies against CCL8, CCL17, CCL20, or CCL28. Sections were counterstained with hematoxylin (top; magnification: 200×). The number of cells was counted. Each symbol represents one donor sample. Horizontal bar = mean; vertical bar = standard deviation. *p < 0.05 and **p < 0.01 according to the Bonferroni method (bottom).