Fig. 1.

Lung transcriptomics of 1-wk-old mice exposed to hyperoxia. Lung tissues of mice exposed to normoxia or hyperoxia for 7 days were analyzed using microarrays. GSEA of microarray data showing significant negatively (A) and positively (B and C) correlated cellular compartmental and biological and molecular function processes, respectively, in the hyperoxia group (FDR < 0.01; P < 0.05). RT-qPCR quantification of ABCC3, BBC3, CDK5R1, GPX2, NTRK2, and PDGFRA mRNA levels in samples from microarray experiments (n = 3 per group) and a separate set of samples (nonarray samples; n = 6 per group) (D). Values are presented as means ± SD *Significant differences between normoxia and hyperoxia groups at P < 0.05 (t-test). FDR, false discovery rate; GSEA, gene set enrichment analysis; RT-qPCR, real-time quantitative PCR; see main text for relevant definitions of genes.