Fig. 3.

Validation of the RPPA results in immunoblot assays: Lung tissues of mice exposed to normoxia or hyperoxia for seven days from RPPA experiments (array samples; n = 3 per group) and a separate set of experiments (nonarray samples; n = 6 per group) were analyzed in immunoblot assays to determine the protein expression levels of p-AMPKα [Thr172], p-AMPKβ1 [Ser108], AMPKα [23A3], PDGFRβ, and SLUG (A). Densitometric analyses wherein p-AMPKα [Thr172] (B), p-AMPKβ1 [Ser108] (C), AMPKα [23A3] (D), PDGFRβ (E), and SLUG (F) band intensities were quantified and normalized to β-actin. The values shown are presented as the mean ± SD *Significant differences between normoxia and hyperoxia groups at P < 0.05 (t-test). AMPK, AMP-activated protein kinase; RPPA, reverse-phase protein array; see main text for relevant definitions of genes.