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Substance P-saporin conjugate (SSP-SAP) in the retrotrapezoid nucleus (RTN) reduces phox2b+/tyrosine hydroxylase (TH)− neurons. Representative photomicrographs of the RTN of rats treated with saline (control; A–C) or SSP-SAP (D–F). In A and D, phox2b-immunoreactivity nuclei appear in red (Cy3), in B and E, TH appears in green (Alexa 488), and in C and F, merge is shown in yellow. Group data (G). *Different from control group, P < 0.05. Scale bar = 100 µm. 7, facial motor nucleus.
