Fig. 3.

Bronchoalveolar lavage (BAL) macrophages (MAC) are highly dependent on mitochondrial respiration for energy production. A: oxygen consumption rate (OCR) can be modulated by addition of glucose and followed by a mitochondrial stress test to yield different bioenergetic parameters. Mitochondrial stress test involves sequential addition of site-specific inhibitors of the electron transport chain: oligomycin (Oligo), FCCP, and rotenone/antimycin A (Rot./Ant. A). B: mitochondrial stress test was performed on MAC sampled from the central airways [induced sputum (IS)] or distal airways (BAL) of normal volunteers. Representative OCR graphs for each sample type are shown. C–G: individual parameters measured from the mitochondrial stress test data for IS and BAL MAC. *P < 0.05 and ****P < 0.0001 for comparison of group means. Shown are means ± SD, IS (n = 13) and BAL (n = 19).