Fig. 7.

Acute lipopolysaccharide (LPS) challenge does not alter the bioenergetics of induced sputum (IS) or bronchial fraction (BF) macrophages. Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of IS (A and B) and BF (C and D) cells were monitored using extracellular flux analysis during exposure to 50 ng/ml LPS for 3–4 h. For BF cells, this exposure was followed by a mitochondrial stress test [oligomycin (Oligo), FCCP, and rotenone/antimycin A (Rot/Ant. A)]. Activity data are expressed normalized for protein content. Representative of n = 3 subjects.