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. 2018 Aug 9;315(5):L752–L764. doi: 10.1152/ajplung.00208.2018

Fig. 9.

Fig. 9.

Exposure to 1,2-naphthoquinone (1,2-NQ) impairs mitochondrial respiration in human bronchoalveolar macrophages (MAC). Effect of 1,2-NQ on induced sputum (IS), bronchoalveolar lavage (BAL), and bronchial fraction (BF) MAC was measured using extracellular flux analysis. Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured at basal conditions, following the addition of 10 μM 1,2-NQ and in response to a mitochondrial stress test [oligomycin (Oligo), FCCP, and rotenone/antimycin A (Rot/Ant. A)]. Data are expressed as the means. OCR (A) and ECAR (B) changes in response to 1,2-NQ addition over a 24-min exposure are shown as a percentage of baseline. Representative OCR and ECAR plots are shown for IS (C and D), BAL (E and F), and BF (G and H), n ≥ 3, means + SD. OCR and ECAR data were normalized to total protein content in the sample. *P < 0.05 and **P < 0.01 between means of DMSO control and 1,2-NQ groups within sample type.