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. 2018 Dec 10;9:1832. doi: 10.3389/fpls.2018.01832

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Copyright © 2018 Verdura, Sales, Ballesteros, Cefalì and Cebrian.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Experimental setup and zygote development into recruits. (A) Fertile thalli and branchlets from natural populations, (B) dispersal bags placed in situ, (C) dispersal bags placed in culture tanks (ex situ), (D) dispersion range capacity under in situ recruitment, and (E) placement of ex situ recruits in the area to be restored. Zygote and embryo development into recruits from ex situ cultures (F–J). (F) Zygotes (1 day), (G) embryos adhered to the substrate by rhizoids (1 week), (H) embryos developing into recruits (1 month, 200–400 μm), (I) first branching of the recruit (1.5 months, 400–600 μm), and (J) fully developed recruits (3 months, 5–15 mm).