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. 2018 Dec 10;9:1768. doi: 10.3389/fphys.2018.01768

FIGURE 1.

FIGURE 1

The dsRNA-processing model is closely related to its nucleotide sequence. (A) REase gene expression levels under different treatments. Fifth instar Asian corn borer larvae were independently injected with 10 μg of dsREase, dsREase-800, dsREase-400A, and dsREase-400B. Four hours later, samples were collected and REase gene expression levels were determined by qRT-PCR. Compared to the dsEGFP treatment, the REase gene can be repressed by the four kinds of dsREase. (B) Processing mode of the four kinds of dsREase in vivo. Fifth instar Asian corn borer larvae were independently injected with 10 μg of dsREase, dsREase-800, dsREase-400A, and dsREase-400B. Four hours later, RNAs were isolated for small RNA sequencing. Small RNAs of 19–25 nt long were re-mapped on the reference sequences (x-axis) to produce the graph. Blue peaks indicate that the small RNAs matched on the sense chain. Red peaks indicate that the small RNAs matched on the anti-sense chain. The x-axis represents the REase sequence, and the y-axis represents the depth of sequencing (amount of mapped small RNA). The three black boxes of dsREase-A, dsREase-B, and dsREase-C were 100 bp sequences in different position for small RNAs analysis. For the statistical results, please see Supplementary Figure 3.