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. 2018 Dec 10;9:3042. doi: 10.3389/fmicb.2018.03042

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Copyright © 2018 Peng, Gao, Lü, Long, Chen, Zhang, Xu, Liu, Wang, Deng, Li and Tao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of dehydrorabelomycin and its BGC by LEXAS screening of the Streptomyces galtieri Sag48 genomic cosmid library using S. lividans GX28 as host. (A) Gene organization of the dehydrorabelomycin BGC in cosmid 8F5. The minimal pks genes are black. (B) HPLC analysis of ethylacetate extracts of fermented cultures. The absorbance was measured at 350 nm. (C) HR-MS spectrum of dehydrorabelomycin isolated from S. lividans LJ1018/8F5. (D) Structure of dehydrorabelomycin. (E) Quantification of the production of dehydrorabelomycin by 8F5-carrying exconjugants in five expression hosts on R3 agar medium.