Fig. 1.

Expression of proximal tubule-enriched genes by HNF4α. HNF4α expression vector was overexpressed in HK-2 (A-C) and HEK293T (D-F) cells for 48 h. Real-time PCR for HNF4A (A and D) and Western blot of HNF4α and γ-tubulin (TUBG) (B and E). Real-time PCR for SLC transporter and megalin (C and F). TBP expression was used as an internal control to normalize expression of the target genes. Relative expression ± S.D. is presented as expression levels based on empty vector-transfected control (n = 3). E, empty vector.