Effect of genistein, daidzein, and trans-oxyresveratrol on PGC-1β-stably overexpressed C2C12 cells in relationship to the expression of the MCAD gene. PGC-1β was stably overexpressed in C2C12 cells (C2C12-PGC-1β). Compared with C2C12 without exogenous PGC-1β, MCAD mRNA levels increased in C2C12-PGC-1β cells. The C2C12-PGC-1β cells were treated with genistein, daidzein, and trans-oxyresveratrol (final concentration 10 µM), for 24 h. Genistein and daidzein significantly increased MCAD mRNA levels. Each value represents mean ± SE (N = 4); ***P < 0.001, **P < 0.01: vs C2C12 (vehicle), ††† P < 0.001, †† P < 0.01: vs C2C12-PGC-1β (vehicle).