Fig. 4.

Effect of genistein, daidzein, and trans-oxyresveratrol on the mitochondrial staining of C2C12-PGC-1β cells A) Control C2C12 cells (without exogenous PGC-1β) and C2C12 cells stably expressing PGC-1β were used (as in Fig. 3). MitoTracker (final concentration 50 nM) was added to the culture medium, and fluorescence was observed with the same exposure time. Compared with control C2C12 cells, C2C12-PGC-1β cells had a marginally increased MitoTracker signal. C2C12-PGC-1β cells were treated with genistein and daidzein, which further enhanced the MitoTracker signal. Typical microscopy images are shown. B) Quantification of images. Relative fluorescence intensity per cells are shown (N = 3). **P < 0.001: vs C2C12 (vehicle), † P < 0.05: vs C2C12-PGC-1β (vehicle).