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PASMC were pre-incubated with 10 μM SB431542 for 1 h or prior transfected with SphK1 siRNA for 24 h or pre-incubated with 10 μM DAPT for 4 h and then stimulated with 10 ng/mL TGF-β1 for an additional 24 h; the proliferation of cells was evaluated by MTT (n = 6 each group). *P < 0.05 vs. control, #P < 0.05 vs. TGF-β1-treated cells, &P < 0.05 vs. control siRNA + TGF-β1-treated group.
