Figure 4.

Deacetylation of XPA increases ATR-mediated phosphorylation of XPA-Ser-196. A, HCT116 ATR^flox/− cells were either transfected with ATR–WT or ATR-435A and were treated with forskolin, VE-821, and/or EX 527, as indicated in A. Cells were mock-treated or exposed to UVB (10 J/m²). Nuclear levels of XPA–pSer-196 were determined by immunoblotting (I.B.). B, XPA CRISPR/Cas9-deleted A375 melanoma cells complemented with either XPA–WT, XPA–K63Q, XPA–K67Q, or XPA–K215Q were pretreated with vehicle or forskolin (10 μm) for 30 min and mock-treated or UVB-irradiated (10 J/m²). Nuclear levels of XPA–pSer-196 were determined by immunoblotting.