Figure 2.

NEAT1 induction by SFN is not dependent on NRF2. A, MCF7 cells were transfected with an siRNA specifically targeting NRF2 (siNRF2) or control siRNA (siCtrl). Twenty four h post-transfection, cells were either left untreated or treated with SFN (20 μm) for 6 h. NEAT1 expression was determined by RT-qPCR as described in Fig. 1. Depletion of NRF2 expression in whole-cell extracts was verified by Western blotting analyses using an NRF2 antibody. The membrane was re-probed with an anti-actin antibody to ensure equal loading. B and C, MCF7 cells were transfected as described in A and subjected to a long-term treatment with SFN (10 μm) for 24 h. The expression of NEAT1 and NEAT1_2 (B) and NQO1 (C) was determined by RT-qPCR. Experiments were performed in triplicate, and the graph is representative of three independent experiments. (**, p ≤ 0.01; *, p, ≤ 0.05; ns, not significant.)