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NEAT1 is induced by heat shock. A and B, MCF7 cells were subjected to heat shock by incubation at 43 °C for 30 min and then returned to 37 °C to recover for the indicated time periods. Activation of HSF1 was verified by shifted migration in Western blotting analyses (A) and by induction of HSP70 mRNA expression (B). C, cells were treated as above, and expression of NEAT1 and NEAT1_2 was assessed by RT-qPCR. (***, p ≤ 0.001; **, p ≤ 0.01; *, p ≤ 0.05.)
