Figure 5.
Differential gene expression analysis shows up-regulation of osteogenic genes in Ezh2-deficient immature chondrocyte cultures. A, differential gene expression analysis of RNA-Seq from WT (Ezh2wt/wt; Col2a1-Cre+/−) and cKOCol2 immature chondrocytes revealed 57 genes that were commonly up-regulated more than 1.4-fold in Ezh2-deficient cultures on days 3, 7, and 14. The analysis was limited to genes with a minimal expression of 0.1 RPKM in the cKO group at each time point. B, the osteogenic extracellular matrix genes Ibsp, Bglap, and Wnt16 were up-regulated in cKOCol2 chondrocytes as well as some nonosteogenic genes such as Cyp27b1, Ebf2, and Otor.