Figure 4.

DCX-ALPA does not rescue the NF endocytosis defect caused by down-regulation of DCX. A, endocytosis of endogenous NF (red) was determined in DIV3 hippocampal neurons expressing shDcx#2-GFP plus rescue plasmid Dcx-R2-ALPA-FLAG (blue). Enlarged views of the red channel are shown below. Green star, transfected cell; arrows, dendrites. Controls are the same as in Fig. 1 and are not shown again. Scale bar, 20 μm. B, DCX-R2-FLAG (cross) and DCX-R2-ALPA-FLAG (red square) are expressed at comparable levels in transfected neurons. n = 25 cells for each plasmid. Transfected cultures were also counterstained with antibody against DCX to compare the extent of overexpression compared with untransfected cell (Fig. S1). C, quantification of NF endocytosis levels. n = 25 cells/condition/experiment. One representative experiment is shown. Error bars, S.E. **, p < 0.001 (ANOVA with post hoc test).