Figure 6.

Divergent phenotypes of DCX-ALPA and DCX-G253D on dendrite growth. A–D, number of dendrites at DIV3 and DIV5 of at least 15-μm length from center of soma was determined for neurons expressing GFP, WT DCX-GFP, or DCX-ALPA-GFP (A and B) or DCX-G253D-GFP (C and D) for DIV3 (A and C) and DIV5 (B and D). One representative experiment is shown (of a total of three independent experiments). n = 50–75 cells/condition in each experiment. The axon was excluded from counts. Transfected cultures were also counterstained with antibody against DCX to compare the extent of overexpression compared with control cells expressing GFP (Fig. S1). For parametric data, we used one-way ANOVA followed by Fisher's least significant difference test. For nonparametric data sets, we used Kruskal–Wallis followed by uncorrected Dunn's multiple-comparison test. Whisker plots show the median as a line, 25–75% as a box, and the data range as whiskers. *, p < 0.05; **, p < 0.001; ****, p < 0.00001; n.s., not significant. Measurements from individual cells are also shown. E–H, representative examples of DIV5 neurons transfected with GFP (E), WT DCX (F), DCX-ALPA (G), or DCX-G253D (H). Dendrites were traced in the right-hand panels in black. The axon is traced in red.