Figure 6.

miR-31-5p inhibits angiogenic activity. A–C, HUVECs were transfected with control miRNA (C), miR-31-5p mimic (M), miR-31-5p inhibitor (I), or DETANO (100 μm), followed by stimulation with TNFα in the presence or absence of l-NAME (1 mm), and cells were then incubated with or without VEGF (10 ng/ml). A, endothelial cell proliferation was determined by [³H]thymidine incorporation assay. B, cell migration was determined by the Boyden chamber assay. C, endothelial cell tube formation was determined on a layer of growth factor-reduced Matrigel. ***, p < 0.001.