Figure 7.

miR-31-5p inhibits trophoblast invasion by suppressing the eNOS/NO/cGMP pathway. A, HUVECs were transfected with control miRNA (C), miR-31-5p mimic (M), or miR-31-5p inhibitor (I), followed by stimulation with TNFα (10 ng/ml), l-NAME (1 mm), ODQ (50 μm), or DMSO (D) as a vehicle for 24 h. The cells were co-cultured with trophoblastic HTR-8/SVneo cells in Transwell plates for 5 h. Trophoblast invasion was determined and quantified using the ImageJ software. B, HTR-8/SVneo cells were transfected with 80 nm control (C) or PKG1 siRNA (si-PKG), followed by treatment with 8-Br-cGMP (300 μm), DETANO, ODQ, or DMSO (D) as a vehicle in Transwell plates for 5 h. Trophoblast invasion was quantified, and PKG1 levels were determined by Western blotting. **, p < 0.01, and ***, p < 0.001.