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. 2018 Dec 16;11:1178636118817738. doi: 10.1177/1178636118817738

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© The Author(s) 2018

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Pseudomonas aeruginosa biofilms and swarms present different chemical signals at different time points. The plate images shows the swarming motility and biofilm colonies on agar-based assays incubated for 8, 24, and 48 hours. Plate assays were analyzed directly by combining CRM (Raman image includes both PQS and AQNO subclasses) and PCA analysis (loading plots and score images for PC1 and PC2) to identify chemical features within the samples. Representative CRM results (n ⩾ 3) collected from biofilm samples and swarm regions both near and away from inoculation center are shown. Scale bars on Raman images represent 10 µm. Loading plots for PC1 and PC2 include features corresponding to tabulated features from Raman spectra of cellular and matrix components (black), PQS/C9-PQS (blue), and AQNOs (HQNO/NQNO; red). Score images of PC1 and PC2 show the distribution of each of the principal components.