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. 2018 Dec 14;24(46):5234–5245. doi: 10.3748/wjg.v24.i46.5234

Table 1.

Primer sequences for quantitative reverse transcription polymerase chain reaction

Primer Primer sequences (5' → 3')
miR-15a F: 5’- ACACTCCAGCTGGGTAGCAGCACATAATGG-3’
R: 10 × miscript universal primer (QIAGEN)
RNU6A F: 5’- CTCGCTTCGGCAGCACA-3’
R: 5’- AACGCTTCACGAATTTGCGT -3’
Cdc42 F: 5’- TTATGACAGATTACGACCGC -3’
R: 5’- CCAACAAGCAAGAAAGGAGT -3’
ZO-1 F: 5’- AACAAGCCAGCAGAGACCTC -3’
R: 5’- CTTCATACATGGGGACGCGA -3’
E-cadherin F: 5’- TCCAGTGAACAACGATGGCA -3’
R: 5’- AATGTACTGCTGCTTGGCCT -3’
GAPDH F: 5’- CCTCCTCACAGTTGCCATGT -3’
R: 5’- CTGGTTGAGCACAGGGTACT -3’

miR-15a: MicroRNA-15a; Cdc42: Cell division cycle 42; ZO-1: Zona occludens-1.