Table 1.
Primer | Primer sequences (5' → 3') |
miR-15a | F: 5’- ACACTCCAGCTGGGTAGCAGCACATAATGG-3’ |
R: 10 × miscript universal primer (QIAGEN) | |
RNU6A | F: 5’- CTCGCTTCGGCAGCACA-3’ |
R: 5’- AACGCTTCACGAATTTGCGT -3’ | |
Cdc42 | F: 5’- TTATGACAGATTACGACCGC -3’ |
R: 5’- CCAACAAGCAAGAAAGGAGT -3’ | |
ZO-1 | F: 5’- AACAAGCCAGCAGAGACCTC -3’ |
R: 5’- CTTCATACATGGGGACGCGA -3’ | |
E-cadherin | F: 5’- TCCAGTGAACAACGATGGCA -3’ |
R: 5’- AATGTACTGCTGCTTGGCCT -3’ | |
GAPDH | F: 5’- CCTCCTCACAGTTGCCATGT -3’ |
R: 5’- CTGGTTGAGCACAGGGTACT -3’ |
miR-15a: MicroRNA-15a; Cdc42: Cell division cycle 42; ZO-1: Zona occludens-1.