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. 2018 Nov 8;9(12):1181–1185. doi: 10.1021/acsmedchemlett.8b00323

Figure 3.

Figure 3

Quantification of mature let-7 levels by qRT-PCR to confirm cellular activity and Lin28-specificity of KCB3602. Changes in miRNA levels after treatment of JAR cells with (a) KCB3602 (≥3 biological replicates) and (b) KCB3613 (3 biological replicates) for 20 h. (c) Effect of KCB3602 on let-7 levels in Lin28 knocked-down JAR cells. Cells were treated with KCB3602 for 20 h (2 biological replicates). (d) Changes in let-7 levels after 20 h treatment of PA-1 human ovarian teratocarcinoma cells with KCB3602 (4 biological replicates). (e) Effect of KCB3602 on let-7 levels in PA-1 cells where Lin28a was knocked out by CRISPR/Cas9-mediated genome editing. Cells were treated with KCB3602 for 20 h (2 technical replicates). Error bars represent standard deviation (*P < 0.05, **P < 0.01, paired two-tailed t test was performed when n ≥ 3). U6 snRNA was used as an endogenous control in all experiments.