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. 2018 Nov 8;9(12):1181–1185. doi: 10.1021/acsmedchemlett.8b00323

Figure 5.

Figure 5

(a) Water-soluble tetrazolium (WST) assay to measure the viability of paclitaxel-resistance JAR cells. Cells were pretreated with KCB3602 or SB1301 for 30 h, and then treated with paclitaxel for 72 h in the absence of those compounds. (b) Effect of KCB3602 or SB1301 on the expression of IMP1 and MDR1 which are associated with drug resistance. Cells were treated with the compound for 30 h. (c) Sphere formation assay to observe the effect of KCB3602 on stem-cell like growth of cancer cells. JAR cells were pretreated with KCB3602 for 24 h, dissociated to single cells, and the same number of viable cells were incubated in sphere forming media for 12 days. Degree of sphere formation was observed under a microscope or (d) measured by WST assay (3 biological replicates, standard deviation, *P < 0.05 from paired two-tailed t test).