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Overexpression of RasGRF1 promotes axonal growth. (A) Neurons were transfected with YFP and RasGRF1 at 1 DIV, then 50 ng/mL of BDNF was added to 2 DIV culture medium, and incubated for 24 h before fixation. Scale bar, 50 µm. (B, C) The effects on axon length (B) and branching (C) were quantitatively analyzed and expressed as means ± SEM of 57 cells in three independent preparations, *p < 0.05; **P < 0.01; ***P < 0.001; ns, not significantly different.
