Fig. 4.

Effects of BDNF on Ser916/898 phosphorylation of RasGRF1. (A) Cortical neurons at 3 DIV were treated with 50 ng/mL of BDNF, 1 mM dbcAMP, and 20 μM forskolin for 30 min. Phosphorylated RasGRF1 (Ser916/898) was analyzed by immunoblotting with the anti-pSer916/898 antibody. (B) Densitometry analysis was performed, and p-RasGRF1/total RasGRF1 ratio was determined. Relative levels of p-RasGRF1/total RasGRF1 compared to vehicle-treated cells are shown. Data are presented as the means ± SEM of four independent preparations, *p < 0.05; **P < 0.01; ***P < 0.001.