Table 2.

Steady‐State Kinetics Parameters of GSTF9 Under Reducing and Oxidizing Conditions

	[GSH]		[CDNB]
	F9red	F9ox	F9red	F9ox
K M	131 ± 9 μM	71 ± 7 μM	1.3 ± 0.1 mM	1.8 ± 0.2 mM
k cat (s−1)	0.85 ± 0.02	0.50 ± 0.01	0.98 ± 0.02	0.72 ± 0.04
k cat/K M (M−1s−1)	6.5 × 103	7.0 × 103	7.5 × 102	4.0 × 102

Kinetic parameters were determined using varying concentrations of GSH or CDNB and the values were plotted using the Michaelis–Menten equation.