Figure 2.

Kinetics of PRDX1 and PRDX2 oxidation by H₂O₂. (A) Time course of the intrinsic fluorescence of 0.25 μM PRDX1 (black line) or 0.25 μM PRDX2 (red line) upon oxidation with 1.25 μM or 1.5 μM H₂O₂, respectively (solid line) and 1 mM H₂O₂ (dashed line) in both cases, at pH 7.4 (traces are the average from 15 runs). (B) The slower phase was fitted to a single exponential function and the first‐order rate constant (k _obs) plotted as a function of H₂O₂ concentration. PRDX1 (black squares) and PRDX2 (red triangles).