Figure 7.

Peroxynitrite effect on PRDX1 depends on the redox state of C83. (a) Different samples of PRDX1 were assayed for peroxidase activity with 50 μM H₂O₂. Peroxynitrite‐treated PRDX1‐C83‐SH, green line; C83‐SS‐R, magenta line; and nontreated PRDX1 (NT, black line). Inset: WB using anti‐nitrotyrosine antibody (samples resolved on a 12% SDS‐PAGE with β‐mercaptoethanol). (b) Peroxidase activity of peroxynitrite‐treated PRDX1‐C83‐SH (right panel) and C83‐SS‐R (left panel) are compared at different H₂O₂ concentration (10 μM, black line; 20 μM, red line; 50 μM, blue line).